Université de Bordeaux
 

Timetable & learning outcomes

The program will integrate, similarly to previous editions, mixed activities with theoretical courses, practical sessions, applied lectures, videos and round table sessions.

Course content will be divided into lectures on methods and instrumentation (incl. latest generation instrumentation and methods) and lectures and applied interactive sessions on case studies (incl. works of art, museum objects and archaeological / palaeontological samples).

Practical sessions will include sample preparation; analysis of peptide, lipid and saccharide fingerprints; bottom up / top down proteomics of ancient proteins; screening and quantification of lipids; mass spectrometry imaging; data processing (sequencing, biological origins, etc.); structural analysis of ancient macromolecules (proteins, lipids, sugars), etc.

Particular attention will be paid to the current questions of cultural heritage stakeholders and to the challenges ahead.

Program

Monday September 26th, 2022

08:00 

Registration

 

08:30

Opening remarks

Caroline Tokarski

 
 

09:00

Lecture - Proteomics basics and application to CH

Caroline Tokarski

10:30

Coffee break

 

11:00

Lecture - ZooMS

Matthew Collins

12:00

Lunch

 

13:30

Lecture - Sample preparation

Leila Birolo

14:30

Experimental sessions:

  • MALDI-TOF-TOF exp. session (Group 2).
    Covered topics: peptides, proteins, polysaccharides
  • LC MS Bottom up proteomics exp. session (Group 1).
    Covered topics: sample preparation, nanoLC-orbitrap settings

 

 17:30

Welcome cocktail 

 

Tuesday September 27th, 2022

08:00

Experimental sessions:

  • MALDI-TOF-TOF exp. session (Group 1).
    Covered topics: peptides, proteins, polysaccharides
  • LC MS Bottom up proteomics exp. session (Group 2).
    Covered topics: sample preparation, nanoLC-orbitrap settings

 

11:00

Lecture - Ambient and imaging

Garry Corthals

12:00

Lunch

 

13:30 

Experimental sessions:

  • MS imaging exp. session (Group 1)
  • LC MS Bottom up proteomics exp. session (Group 2)
    Covered topics: data treatment for protein ID, species ID

 

15:30

Coffee break

 

16:00

Experimental sessions:

  • MS imaging exp. session (Group 2)
  • LC MS Bottom up proteomics exp. session (Group 1)
    Covered topics: data treatment for protein ID, species ID

 

18:00

Free time


Wednesday September 28th, 2022

08:30

Lecture - Chemistry in cultural heritage samples

Ilaria Bonaduce

09:30

Experimental session:

  • Structural analysis
    Covered topics: XL, top down, native and IMS MS


13:00

Lunch

 

14:00


Wrap-up session


 

14:30

Social program (cruise and cocktail)

 

18:00

Free time


Thursday September 29th, 2022

08:00

Registration


08:30

Opening remarks


09:00

Welcome remark and Introduction to the collection of Aquitaine Museum & associated research

Laurent Védrine

Chairman: Caroline Tokarski

09:20

Study and conservation of works of art at the C2RMF and investment in European infrastructures

Victor Etgens

Chairman: Caroline Tokarski

09:40

Session 1: Paint binders

  • Analysis of binders and additives in Talens ETA and Rembrandt tempera paints
  • A mass spectrometric study of the oil paint curing using methyl linoleate as a model binder
  • A retrospective and critical review of binding media analysis in wall paintings

Inez Dorothé van der Werf

Silvia Pizzimenti

Joy Mazurek

Chairman: Klaas Jan van den Berg

10:40

Posters: flash presentation**

Chairman: Catherine Gilbert

11:00

Coffee break & posters*

 

11:30

Ancient proteins - future directions in the past

Matthew Collins

Chairman: Caroline Tokarski

12:30

Lunch

 

13:30

Aquitaine Museum tour guided by the Director 

 Laurent Vedrine

14:15

Computational paleoproteomics

Juergen Cox

Chairman: Francesca Galluzzi

14:45

Session 2: Protein analysis

  • Identifying emulsion-cured leather in museum collections using proteomics

  • Discrimination of plant gum polysaccharides using MALDI-MS: developments, limitations, and exploration of a complementary proteomics approach

  • Bloody proteins – an ongoing quest to identify 2000-year-old proteinaceous residues on ceramics from the Late Iron Age and Roman Period

Aleksandra Popowich

Clara Granzotto

Tania F.M. Oudemans

Chairman: Francesca Galluzzi

15:45

Coffee break & posters*


16:00

Poster session**


16:20

Chemistry of Paint Film Formation

Ilaria Bonaduce

Chairman: Klaas Jan van den Berg

16:50

Departure by bus (social program)


Friday September 30th, 2022

08:50

Opening remarks


  

09:00

Welcome remark and Introduction to the history of the Grand Theather

Emmanuel Hondré

09:20

Omics in the Museum World: grit, education, communication, and ethics at the Met

Julie Arslanoglu

Chairman: Ken Sutherland

09:50

Session 3: Lacquers

  • Identification and differentiation of essential oils used as solvents in paints and lacquer works
  • Blended Thitsiol/Urushiol Asian lacquers in cultural heritage: new advances in understanding their curing, ageing, and photo-ageing processes by THM-GC/MS
  • Differentiation between primary and secondary pyrolysis reactions to improve the identification of Asian lacquer macromolecules

Patrick Dietemann

Valentina Pintus

Jonas Veenhoven

Chairman: Ester Ferreira

10:50

Coffee break & posters*


11:10

Session 4: Sampling and minimally invasive methods

  • Minimally invasive proteomics workflow to determine the species of origin of ivory objects
  • New GC-MS sampling approaches at the Rijksmuseum: current challenges and future perspectives

Catherine Gilbert

Alba Alvarez-Martin

Chaiman: Christopher Maines

11:50

Session 5: Synthetic polymers

  • Characterization of historical PMMA sheets used by two Portuguese artists by a comparative study of three analytical methods: EGA-MS, TD-GC/MS and Py-GC/MS
  • PVC “Kunststoffschule” A valuable resource in the research of technical and material development of the PVC industry in Germany 1950-1970. Establishing a link between composition and end use

Anna Micheluz

Ester S.B. Ferreira

Chairman: Ken Sutherland

12:30

Lunch & posters*


13:30

Session 6: Technological and methodological advances

  • Rapid characterization of organic material sampled from surfaces of art works and other cultural heritage related objects by atmospheric solids analysis probe – high-resolution mass spectrometry
  • Top-Down MS: the next frontier in MS proteomic analysis of cultural heritage samples?
  • Native proteins relevant to cultural heritage at nanomolar and picoliter quantities using triboelectric nanogenerator and ion mobility-mass spectrometry

Wim Genuit

Vaclav Krupicka

Daniel D. Vallejo

Chairman: Aleksandra Popowich

14:30

Archaeometry, mass spectrometry and 3D technologies: how can they match?

Remy Chapoulie

Chairman: Nicolas Desbenoit

15:00

Coffee break


15:20

Round table - Contribution of modern mass spectrometry to major questions of cultural

Klaas Jan van den Berg

Christopher Maines

Ken Sutherland

Ester Ferreira

Julie Arslanoglu

Chairman: Caroline Tokarski

16:00

MaSC Business Meeting chaired by MaSC committee and Closing Remark


* The presence of poster authors not necessarily required
** The presence of poster authors required

Expertise upon completion

The summer school offers a unique opportunity to develop strong knowledge in advanced mass spectrometry within a program which links analytical chemists, physicists, experts in formulation, computational modeling experts, conservators, restorers and archaeologists.

A certificate of participation will be awarded to students upon completion of the course.












Program may be subject to change. 



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