Université de Bordeaux

Timetable & learning outcomes

The program will integrate, similarly to the summer school organized in 2019, mixed activities with theoretical courses, practical sessions, applied lectures, videos and round table sessions.

Course content will be divided into lectures on methods and instrumentation (incl. latest generation instrumentation and methods) and lectures and applied interactive sessions on case studies (incl. works of art, museum objects and archaeological / palaeontological samples). Practical sessions will include sample preparation; analysis of peptide, lipid and saccharide fingerprints; bottom up / top down proteomics of ancient proteins; screening and quantification of lipids; mass spectrometry imaging; data processing (sequencing, biological origins, etc.); structural analysis of ancient macromolecules (proteins, lipids, sugars), etc. Particular attention will be paid to the current questions of cultural heritage stakeholders and to the challenges ahead.

Tentative program

Wednesday June 16th, 2021

   

Virtual registration

 
   

Opening remarks

 
   

Opening lecture - Mass spectrometry applied to cultural heritage: current achievements and future challenges

 
   

Virtual coffee break

 
   

Lecture - Chemistry and art at the Metropolitan Museum of Art [New York]: natural organic polymer investigations

and

Lecture - Research at the National Museum of Prehistory [Les Eyzies] and international principles of resource preservation and knowledge development in archaeology

 
   

Lunch

 
   

180 second presentations (10 attendees)

 
   

Lecture - Sample preparation and minimally invasive methods for proteomics: principles and case studies applied to artworks and archaeological samples

 
   

Best practices (videos, on-line practicals)

  • Peptides, proteins 

  • Lipids, sugars, other polymers

  • Sampling and storage

 
   

Virtual icebreaker

 

Thursday June 17th, 2021

   

Lecture - Palaeoproteomics to unleash the study of human history

 
   

Lecture - Proteomics / Palaeoproteomics: workflow, analytical designs

 
   

Virtual coffee break

 
   

Practicals - methods

  • Bottom up methods: LC MS, ZooMS

  • Top down methods: MALDI-based, LC MS

  • Challenges: deep proteome, screening / automation, questioning the unknown part of datasets, etc.

 
   

Summary - general discussion

 
   

Lunch

 
   

180 second presentations (10 attendees)

 
   

Lecture - Authentication

 
   

Lecture - Bioinformatics of ancient proteins

 
   

Praticals - applied case studies

  • Authentication

  • Data processing, protein modification / substitution

  • Protein networks, crosslinkings, degradation

 
   

Summary - general discussion

 
   

Lecture - Ethics

 

Friday June 18th, 2021

   

Virtual breakfast

 
   

Lecture - MS imaging fundamentals and MSI applied to cultural heritage 

 
   

Lecture - Lipid degradations in heritage material using TOF-SIMS and LC-MS/MS

 
   

Virtual coffee break

 
   

Praticals - applied case studies

  • MS imaging

  • Lipid analysis

 
   

Lunch

 
   

Lecture - GC-MS and PyGC-MS applied to the study of organic material -proteins, lipids, polysaccharides- in artworks and archaeological samples

 
   

Lecture - Ambient MS and SAWN applied to heritage

 
   

Virtual coffee break

 
   

Award for the best 180 second presentation

 
   

Round table: Contribution of Modern Mass Spectrometry to Major Questions of Cultural Heritage (virtual cheese and wine session)

 
   

Virtual escape game

 
   

Concluding remarks

 

Expertise upon completion

The summer school offers a unique opportunity to develop strong knowledge in advanced mass spectrometry within a program which links analytical chemists, physicists, experts in formulation, computational modeling experts, conservators, restorers and archaeologists.

A certificate of participation will be awarded to students upon completion of the course.

Program may be subject to change. 



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